6J6Y
FGFR4 D2 - Fab complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-25 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.218, 119.165, 105.099 |
| Unit cell angles | 90.00, 97.29, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.150 |
| R-factor | 0.1963 |
| Rwork | 0.193 |
| R-free | 0.25820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.383 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.410 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.153 | 1.432 |
| Rmeas | 0.209 | 1.939 |
| Rpim | 0.142 | 1.298 |
| Total number of observations | 408438 | |
| Number of reflections | 74039 | 4586 |
| <I/σ(I)> | 6.6 | 0.9 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.991 | 0.321 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 1.9M ammonium sulfate, 0.1M sodium acetate, pH 4.5 |
