6J6W

Mutant K23N of heat shock factor 4-DBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-06-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	P 2 21 21
Unit cell lengths	44.632, 65.047, 80.556
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.169 - 1.693
R-factor	0.1727
Rwork	0.171
R-free	0.20520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.207
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.170	1.730
High resolution limit [Å]	1.690	1.690
Number of reflections	26197
<I/σ(I)>	24.1
Completeness [%]	99.4
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		295	sodium nitrate