Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.660, 95.960, 97.930 |
| Unit cell angles | 90.00, 92.15, 90.00 |
Refinement procedure
| Resolution | 29.187 - 2.624 |
| R-factor | 0.2791 |
| Rwork | 0.278 |
| R-free | 0.30090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sqd |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.619 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.770 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.571 | 1.687 |
| Number of reflections | 52627 | 7642 |
| <I/σ(I)> | 9.72 | |
| Completeness [%] | 99.1 | 98.9 |
| Redundancy | 3.7 | 3.6 |
| CC(1/2) | 0.764 | 0.429 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291.16 | 0.1M NaAc at pH 4.5, 0.1M NaCl, 40% PEG 400 |
