6J62
Crystal structure of mISG15/NS1B complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-03-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.113, 37.766, 72.531 |
Unit cell angles | 90.00, 101.35, 90.00 |
Refinement procedure
Resolution | 35.580 - 2.486 |
R-factor | 0.2077 |
Rwork | 0.204 |
R-free | 0.27790 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.972 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.580 | 2.570 |
High resolution limit [Å] | 2.480 | 2.480 |
Number of reflections | 10221 | |
<I/σ(I)> | 14.4 | |
Completeness [%] | 98.0 | |
Redundancy | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M magnesium chloride, 0.1M Na-HEPES, pH 7.0, 13 %(w/v) PEG 4000. |