6J4O
Structural basis of tubulin detyrosination by vasohibins-SVBP enzyme complex and functional implications
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-25 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 83.140, 123.248, 195.171 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.310 - 2.300 |
R-factor | 0.1874 |
Rwork | 0.184 |
R-free | 0.22390 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.047 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL2Map |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.134 | 1.000 |
Rmeas | 0.146 | |
Rpim | 0.058 | 0.459 |
Number of reflections | 25255 | 1254 |
<I/σ(I)> | 12.2 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.5 | 5.6 |
CC(1/2) | 0.691 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8.2 | 293 | 1.6 M sodium/potassium phosphate, pH 8.2 |