6J4G

Crystal structure of the AtWRKY33 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978
Spacegroup name	P 65 2 2
Unit cell lengths	97.900, 97.900, 94.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.473 - 3.000
R-factor	0.2079
Rwork	0.204
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ayd
RMSD bond length	0.010
RMSD bond angle	1.168
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.474	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.175	0.800
Rmeas	0.179
Rpim	0.035	0.470
Number of reflections	5684	550
<I/σ(I)>	13
Completeness [%]	98.8	100
Redundancy	24.4	24.1
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	8% v/v Ethylene glycol, 0.1M HEPES/Sodium hydroxide pH 7.5, 10% PEG 8000