Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.204, 53.173, 64.271 |
| Unit cell angles | 90.00, 99.92, 90.00 |
Refinement procedure
| Resolution | 27.002 - 1.900 |
| R-factor | 0.2168 |
| Rwork | 0.214 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g8c |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.913 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.320 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.075 | 0.500 |
| Number of reflections | 17284 | 833 |
| <I/σ(I)> | 24 | 4.72 |
| Completeness [%] | 98.3 | 96.9 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 40% PEG 400, 0.1M Tris-HCl pH 9.0, protein 10 mg/mL |
