6J3X

The Structure of Maltooligosaccharide-forming Amylase from Pseudomonas saccharophila STB07 with Maltotriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.978910
Spacegroup name	P 21 21 21
Unit cell lengths	46.320, 64.650, 169.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.470 - 1.620
R-factor	0.1649
Rwork	0.164
R-free	0.18610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iwk
RMSD bond length	0.010
RMSD bond angle	1.130
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.470	4.670
High resolution limit [Å]	1.620	1.620
Number of reflections	65292
<I/σ(I)>	1.38
Completeness [%]	99.6
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1 M ammonium sulfate, 0.02 M Tris-NCl