6J36

crystal structure of Mycoplasma hyopneumoniae Enolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97892
Spacegroup name	I 4
Unit cell lengths	193.154, 193.154, 63.943
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.289 - 2.301
R-factor	0.1885
Rwork	0.186
R-free	0.22570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mks
RMSD bond length	0.008
RMSD bond angle	0.886
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.289	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.108	0.597
Number of reflections	52577	39272
<I/σ(I)>	14.2
Completeness [%]	99.8
Redundancy	4.6
CC(1/2)	0.997	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	291	0.056 M sodium phosphate monobasic monohydrate, 1.344 M potassium phosphate dibasic, pH=8.4