6J0Z
Crystal structure of AlpK
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-11 |
Detector | APEX II CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 89.882, 89.882, 260.737 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.235 - 2.889 |
R-factor | 0.2584 |
Rwork | 0.256 |
R-free | 0.30080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4x4j |
RMSD bond length | 0.009 |
RMSD bond angle | 1.491 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.240 | 2.992 |
High resolution limit [Å] | 2.889 | 2.889 |
Rmerge | 0.013 | 0.109 |
Number of reflections | 14697 | 1204 |
<I/σ(I)> | 25.89 | 5.58 |
Completeness [%] | 95.0 | 83.98 |
Redundancy | 2 | 2 |
CC(1/2) | 1.000 | 0.973 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289.15 | 0.01M magnesium sulfate heptahydrate, 0.1M sodium Cacodylate, 0.8M ammonium sulfate |