6J0T
The crystal structure of exoinulinase INU1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-17 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.00822 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 172.650, 172.650, 172.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.840 - 2.800 |
| R-factor | 0.202 |
| Rwork | 0.198 |
| R-free | 0.27150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eqv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.097 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.840 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.098 | 0.256 |
| Number of reflections | 32347 | 3177 |
| <I/σ(I)> | 11.7 | 3 |
| Completeness [%] | 99.9 | 99.94 |
| Redundancy | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 18% PEG4000, 0.1 M sodium cacodylate pH6.0, 0.2 M MgCl2 |
