6J0Q
Crystal structure of P domain from GII.11 swine norovirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 76.237, 76.237, 241.799 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.18154 |
| Rwork | 0.180 |
| R-free | 0.21627 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pun |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.211 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 1.998 | 2.000 |
| Rmerge | 0.140 | 0.489 |
| Number of reflections | 49356 | 2396 |
| <I/σ(I)> | 30 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.7 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100mM Tris (pH 8.0), 16% (w/v) polyethylene glycol 3350, 6% (v/v) tacsimate (pH 8.0) |
