6J07

Crystal structure of human TERB2 and TERB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 64 2 2
Unit cell lengths	126.900, 126.900, 90.429
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.959 - 3.298
R-factor	0.291
Rwork	0.288
R-free	0.33690
Structure solution method	SAD
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.420
High resolution limit [Å]	3.298	7.100	3.300
Rmerge	0.076	0.042	0.843
Rmeas	0.079	0.044	0.885
Rpim	0.021	0.012	0.257
Number of reflections	6870	769	638
<I/σ(I)>	5.9
Completeness [%]	99.6	99.5	96.1
Redundancy	14.7	13.2	9.9
CC(1/2)		0.999	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	9	291	0.1 M Bicine pH 9.0, 2% (v/v) 1,4-Dioxane, 4% (w/v) Polyethylene glycol 20000
1	EVAPORATION	9	291	0.1 M Bicine pH 9.0, 2% (v/v) 1,4-Dioxane, 4% (w/v) Polyethylene glycol 20000