6J02

Crystal structure of the SRCR domain of mouse SCARA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2017-06-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 1
Unit cell lengths	39.274, 42.881, 45.469
Unit cell angles	91.03, 110.81, 117.35

Refinement procedure

Resolution	25.091 - 1.803
R-factor	0.1825
Rwork	0.180
R-free	0.21010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1by2
RMSD bond length	0.009
RMSD bond angle	0.903
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.090	0.316
Number of reflections	21345	4117
<I/σ(I)>	19.758
Completeness [%]	96.6
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	polyethylene glycol 300, polyethylene glycol 8000, Glycerol, calcium, Tris (pH 8.5)