6IZY

The RNA-dependent RNA polymerase domain of dengue 2 NS5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-03
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	I 4 3 2
Unit cell lengths	237.350, 237.350, 237.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.958 - 2.110
R-factor	0.1954
Rwork	0.195
R-free	0.22110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2j7u
RMSD bond length	0.005
RMSD bond angle	0.746
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.140	2.160
High resolution limit [Å]	2.110	2.110
Number of reflections	65159	3092
<I/σ(I)>	28.2
Completeness [%]	95.7
Redundancy	13.7
CC(1/2)	0.999	0.398

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.6 M ammonium sulfate, 100 mM MES-NaOH (pH 6.2-6.5), and 10 mM CoCl2