6IZH
Crystal structure of deaminase AmnE from Pseudomonas sp. AP-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 217 |
| Detector technology | CCD |
| Collection date | 2018-10-05 |
| Detector | RIGAKU |
| Wavelength(s) | 0.9778 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.871, 54.781, 134.594 |
| Unit cell angles | 90.00, 101.89, 90.00 |
Refinement procedure
| Resolution | 49.511 - 1.754 |
| R-factor | 0.1588 |
| Rwork | 0.157 |
| R-free | 0.19490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dyy |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.980 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.511 | 1.810 |
| High resolution limit [Å] | 1.754 | 1.760 |
| Rmerge | 0.901 | 0.535 |
| Rpim | 0.044 | 0.244 |
| Number of reflections | 120742 | 19456 |
| <I/σ(I)> | 28.25 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.6 | 293.15 | CAPSO,Magnesium chloridehexahydrate,PEG4000. |
