6IZ6

Crystal Structure Analysis of TRIC counter-ion channels in calcium release

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-20
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.7712
Spacegroup name	F 4 3 2
Unit cell lengths	250.110, 250.110, 250.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.134 - 3.293
R-factor	0.3276
Rwork	0.326
R-free	0.35420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iz5
Data reduction software	XDS
Data scaling software	Aimless (0.7.2)
Phasing software	PHENIX
Refinement software	PHENIX (1.14rc2_3191)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.134	48.130	3.550
High resolution limit [Å]	3.290	8.710	3.290
Rmerge	0.213	0.088	6.966
Rmeas	0.214	0.088	7.047
Rpim	0.023	0.009	1.043
Number of reflections	9924	679	1812
<I/σ(I)>	15.7
Completeness [%]	92.6	99.4	84.7
Redundancy	82.8	92.7	44.2
CC(1/2)	1.000	1.000	0.583

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		278	PEG 350 MME, CaCl2, MES PH6.5