6IZ1

Crystal Structure Analysis of a Eukaryotic Membrane Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-08
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.9792
Spacegroup name	P 63 2 2
Unit cell lengths	127.348, 127.348, 101.801
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.487 - 2.399
R-factor	0.2187
Rwork	0.217
R-free	0.25670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iyu
Data reduction software	XDS
Data scaling software	Aimless (0.7.2)
Phasing software	PHENIX
Refinement software	PHENIX (1.14rc2_3191)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.490	48.490	2.490
High resolution limit [Å]	2.400	8.970	2.400
Rmerge	0.140	0.035	5.454
Rmeas	0.142	0.035	5.522
Rpim	0.023	0.007	0.858
Total number of observations	688445	13453	80857
Number of reflections	17876	457	1996
<I/σ(I)>	23.1	108.2	1.1
Completeness [%]	90.9	99.4	99.9
Redundancy	38.5	29.4	40.5
CC(1/2)	1.000	1.000	0.482

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		278	PEG MME 2000