6IZ0

Crystal Structure Analysis of a Eukaryotic Membrane Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-31
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.9792
Spacegroup name	P 63 2 2
Unit cell lengths	127.049, 127.049, 101.448
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.587 - 2.300
R-factor	0.2198
Rwork	0.219
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iyu
Data reduction software	XDS
Data scaling software	Aimless (0.7.2)
Phasing software	PHENIX
Refinement software	PHENIX (1.14rc2_3191)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.590	41.590	2.270
High resolution limit [Å]	2.200	9.080	2.200
Rmerge	0.189	0.023	6.754
Rmeas	0.191	0.024	6.848
Rpim	0.030	0.004	1.113
Total number of observations	1001482
Number of reflections	24924	417	2029
<I/σ(I)>	23.9
Completeness [%]	99.6	96.6	96.1
Redundancy	40.2	31.9	36.5
CC(1/2)	1.000	1.000	0.359

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		278	PEG MME 2000