6IYA

Structure of the DNA binding domain of antitoxin CopASO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 2 21
Unit cell lengths	58.541, 60.949, 105.098
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.220 - 3.000
R-factor	0.2493
Rwork	0.247
R-free	0.29810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gpe
RMSD bond length	0.002
RMSD bond angle	0.583
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.088	0.782
Rmeas	0.098	0.873
Rpim	0.042	0.378
Number of reflections	7302	729
<I/σ(I)>	15.7	2.2
Completeness [%]	90.4	92.2
Redundancy	4.8	4.6
CC(1/2)	0.974	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		279	0.2 M Sodium formate, 20% w/v Polyethylene glycol 3350