6IXO

Apo structure of Myo2-GTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	50.660, 72.164, 168.324
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.081 - 1.901
R-factor	0.1751
Rwork	0.174
R-free	0.20630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f6h
RMSD bond length	0.014
RMSD bond angle	1.259
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.900	5.160	1.900
Rmerge	0.106	0.053	1.905
Rmeas	0.111	0.056	1.988
Rpim	0.032	0.017	0.562
Number of reflections	49545	2696	2455
<I/σ(I)>	5.7
Completeness [%]	100.0	99.8	100
Redundancy	11.6	10.7	12.1
CC(1/2)		0.998	0.764

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	1 M potassium chloride, 1 M ammonium sulfate and 0.1 M HEPES pH 7.0