6IX1

Structure of 2S albumin seed protein from Dolichos

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-18
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.88560
Spacegroup name	P 31
Unit cell lengths	65.158, 65.158, 118.167
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.410 - 1.280
R-factor	0.13262
Rwork	0.128
R-free	0.16980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3oyo
RMSD bond length	0.020
RMSD bond angle	2.169
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (v1.8.2-1309)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.410	1.294
High resolution limit [Å]	1.280	1.280
Rmerge	0.054	0.432
Rmeas	0.067
Rpim	0.039	0.341
Number of reflections	140457	13099
<I/σ(I)>	15.6
Completeness [%]	97.0
Redundancy	2.8
CC(1/2)	0.986	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	295	Sodium acetate trihydrate, Ammonium sulphate