6IWM

Structural insight into probable lipid transfer mechanism of non-specific lipid transfer protein via intermediate structures in Solanum melongena

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-02-24
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 61
Unit cell lengths	87.036, 87.036, 50.342
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.130 - 1.980
R-factor	0.1874
Rwork	0.186
R-free	0.21720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tvi
RMSD bond angle	0.930
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2747: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.010
High resolution limit [Å]	1.980	1.980
Rmerge	0.064	0.639
Number of reflections	15253	1507
<I/σ(I)>	38.4	2.42
Completeness [%]	99.9	99.9
Redundancy	8.4	7.6
CC(1/2)	0.955	0.748

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295.15	0.1M HEPES pH 7.0, 2M Ammonium sulfate