6IWK
The Structure of Maltooligosaccharide-forming Amylase from Pseudomonas saccharophila STB07
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-21 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97892 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.193, 64.768, 168.736 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.072 - 1.501 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.20710 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.821 |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.072 | 10.000 |
High resolution limit [Å] | 1.501 | 2.000 |
Number of reflections | 81383 | |
<I/σ(I)> | 1.37 | |
Completeness [%] | 99.4 | |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1 M ammonium sulfate, 20 mM Tris-HCl |