6IW8

Crystal structure of Importin-alpha and wild-type GM130

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-24
Detector	RAYONIX MX300HE
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	75.399, 78.628, 90.318
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
Rwork	0.227
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iq1
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	13194	1355
<I/σ(I)>	16.7
Completeness [%]	98.7	91.7
Redundancy	5.5
CC(1/2)	0.996	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	0.1M Sodium malonate, 12% w/v Polyethylene glycol 3350 (pH 7.0)