6IW6
Crystal structure of the Lin28-interacting module of human TUT4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 113.270, 127.830, 168.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.963 - 2.402 |
R-factor | 0.2113 |
Rwork | 0.210 |
R-free | 0.23710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5w0n |
RMSD bond length | 0.010 |
RMSD bond angle | 0.998 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.12-2829_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.963 | 19.963 | 2.460 |
High resolution limit [Å] | 2.400 | 10.740 | 2.400 |
Rmerge | 0.190 | 0.033 | 2.372 |
Rmeas | 0.194 | 0.034 | 2.419 |
Total number of observations | 1288392 | ||
Number of reflections | 48018 | 600 | 3482 |
<I/σ(I)> | 18.11 | 75.89 | 1.69 |
Completeness [%] | 99.9 | 98.2 | 99.3 |
Redundancy | 26.831 | 21.363 | 26.144 |
CC(1/2) | 0.999 | 1.000 | 0.762 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 100 mM Hepes-NaOH, pH 7.0, 20% (w/v) PEG 3350, 3% MPD, 200 mM Ammonium Citrate and 3% (w/v) 1,5-Diaminopentane dihydrochloride |