6IW3

High resolution structure of Dvl2-DIX Y27W/C80S mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-18
Detector	RAYONIX MX225HE
Wavelength(s)	0.9000
Spacegroup name	P 61
Unit cell lengths	44.470, 44.470, 87.650
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.500 - 1.640
R-factor	0.2026
Rwork	0.200
R-free	0.22920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wip
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.680
High resolution limit [Å]	1.640	1.640
Rmerge	0.133	2.300
Rmeas	0.136	2.370
Number of reflections	12127	873
<I/σ(I)>	20.3
Completeness [%]	100.0	100
Redundancy	19.7	13.9
CC(1/2)	0.999	0.511

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	20% (w/v) PEG 6000, Tris-HCl pH 8.0, 200 mM lithium chloride