6IW0
Crystal structure of 5A ScFv in complex with YFV-17D sE in postfusion state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | H 3 2 |
Unit cell lengths | 103.991, 103.991, 368.675 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.464 - 3.600 |
R-factor | 0.2792 |
Rwork | 0.277 |
R-free | 0.33590 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.881 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.730 |
High resolution limit [Å] | 3.600 | 3.600 |
Number of reflections | 8668 | |
<I/σ(I)> | 7.7 | |
Completeness [%] | 99.8 | |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 291 | 0.1 M Sodium malonate pH 4.0, 12% w/v Polyethylene glycol 3350 |