6IVJ

Crystal structure of a membrane protein G18A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	114.119, 160.027, 161.784
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.760 - 2.770
R-factor	0.2168
Rwork	0.215
R-free	0.24434
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.614
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	161.780	2.820
High resolution limit [Å]	2.770	2.770
Rmerge	0.010	2.310
Rpim	0.004	1.016
Number of reflections	75940	4260
<I/σ(I)>	15.5	0.8
Completeness [%]	99.5	95.5
Redundancy	6.7	5.9
CC(1/2)		0.333

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.05 M zinc acetate, 6% v/v ethylene glycol, 0.1 M sodium cacodylate, pH 6.0, 6.6 % w/v PEG 8000