6IV9
the Cas13d binary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-05-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.224, 136.481, 147.283 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.110 - 1.860 |
R-factor | 0.1712 |
Rwork | 0.170 |
R-free | 0.19560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6iv8 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.735 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.110 | 1.960 |
High resolution limit [Å] | 1.860 | 1.890 |
Rpim | 0.029 | 0.352 |
Number of reflections | 121127 | 5918 |
<I/σ(I)> | 21 | 3.6 |
Completeness [%] | 98.9 | 98.8 |
Redundancy | 13.5 | 14.1 |
CC(1/2) | 0.999 | 0.886 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | (8% (v/v) Tacsimate (pH 7.0), 17% (w/v) Polyethylene glycol 3350) |