6IV7
The crystal structure of a SAM-dependent enzyme from aspergillus flavus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97915 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 160.556, 62.225, 113.354 |
Unit cell angles | 90.00, 113.06, 90.00 |
Refinement procedure
Resolution | 41.881 - 1.937 |
R-factor | 0.2041 |
Rwork | 0.202 |
R-free | 0.23770 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | Aimless (0.6.2) |
Phasing software | PHENIX (1.14_3260) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.910 | 1.940 |
High resolution limit [Å] | 1.937 | 1.940 |
Rmerge | 0.092 | 0.869 |
Rmeas | 0.109 | |
Rpim | 0.058 | |
Number of reflections | 72748 | 11076 |
<I/σ(I)> | 2.15 | |
Completeness [%] | 94.8 | 99.4 |
Redundancy | 6.7 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.05M CaCl2, 0.1M Bis-Tris PH 6.5, 30% mPEG 550 |