6IV0

Crystal structure of a bacterial Bestrophin homolog from Klebsiella pneumoniae with a mutation I180A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	113.573, 159.905, 161.261
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.377 - 2.900
R-factor	0.2022
Rwork	0.201
R-free	0.23790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.389
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.377	3.004
High resolution limit [Å]	2.900	2.900
Number of reflections	65655
<I/σ(I)>	17.63
Completeness [%]	99.8
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.05M zinc acetate, 6% v/v ethylene glycol, 0.1M sodium cacodylate, pH 6.0, 6.6 % w/v PEG 8000