6IUH

Crystal structure of GIT1 PBD domain in complex with Liprin-alpha2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	88.200, 38.617, 99.134
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.567 - 1.800
R-factor	0.2285
Rwork	0.227
R-free	0.26680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jx0
RMSD bond length	0.003
RMSD bond angle	0.618
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.098	0.066	0.842
Rmeas	0.110	0.076	0.930
Rpim	0.050	0.036	0.390
Number of reflections	32467	1785	1606
<I/σ(I)>	5.7
Completeness [%]	99.2	98.5	100
Redundancy	5.3	4.7	5.6
CC(1/2)		0.994	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.2M Potassium iodide, 0.1M MES pH 6.5, 25% w/v PEG 4000