6IU0
Peroxiredoxin from Thermococcus kodakaraensis (0Cys mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1 |
| Spacegroup name | P 6 |
| Unit cell lengths | 141.307, 141.307, 48.531 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.020 - 2.380 |
| R-factor | 0.21538 |
| Rwork | 0.213 |
| R-free | 0.26995 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w6g |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.929 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.420 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Number of reflections | 22561 | |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M HEPES-NaOH (pH 7.5), 20% PEG4000, and 8% 2-propanol |
