6ITG
a new alginate lyase (PL6) from Vibrio splendidus OU02
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2017-12-25 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1 |
Spacegroup name | P 31 |
Unit cell lengths | 106.327, 106.327, 100.575 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.420 |
R-factor | 0.12689 |
Rwork | 0.125 |
R-free | 0.16015 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gkd |
RMSD bond length | 0.016 |
RMSD bond angle | 1.631 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.470 |
High resolution limit [Å] | 1.420 | 1.420 |
Number of reflections | 216544 | |
<I/σ(I)> | 20.6 | |
Completeness [%] | 99.9 | |
Redundancy | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | pH7.0, 2.4M Sodium malonate |