6ISG

Structure of 9N-I DNA polymerase incorporation with dG in the active site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	298
Detector technology	CCD
Collection date	2016-11-25
Detector	RAYONIX MX300-HS
Wavelength(s)	0.9998
Spacegroup name	I 2 3
Unit cell lengths	208.078, 208.078, 208.078
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.810 - 3.401
R-factor	0.25472
Rwork	0.252
R-free	0.30168
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f8x
RMSD bond length	0.013
RMSD bond angle	1.290
Data reduction software	XDS (November 1, 2016 built 20161205)
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.810	3.522
High resolution limit [Å]	3.401	3.401
Rmerge	0.027	0.476
Number of reflections	20707	2046
<I/σ(I)>	8.35
Completeness [%]	99.9
Redundancy	2
CC(1/2)	0.999	0.585

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Sodium Acetate pH4.6, MPD, Glycerol, CaCl2