6ISE
Crystal structure of AMPPNP bound CK2 alpha from C. neoformans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.877, 95.594, 93.300 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 47.840 - 2.800 |
| R-factor | 0.19808 |
| Rwork | 0.196 |
| R-free | 0.22974 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lp4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.556 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.075 | 0.161 |
| Number of reflections | 18151 | |
| <I/σ(I)> | 18.7 | |
| Completeness [%] | 97.8 | |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290 | 0.2 M lithium sulfate, 0.1 M Tris pH 8.5, 30 %(w/v) PEG 4000, 30%(w/v) dextran sulfate sodium salt, 2 mM MgCl2, 5 mM AMPPNP |
