6IRX

Crystal structure of the zebrafish cap-specific adenosine methyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	71.545, 84.132, 93.084
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.033 - 2.000
R-factor	0.1923
Rwork	0.191
R-free	0.21540
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless (0.7.2)
Phasing software	SHELXD
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.033	47.030	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.135	0.085	1.054
Rmeas	0.141	0.089	1.098
Rpim	0.040	0.026	0.303
Number of reflections	38694	511	2822
<I/σ(I)>	10.1
Completeness [%]	100.0	99.6	100
Redundancy	12.4	11	12.9
CC(1/2)	0.996	0.990	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	11% PEG 3350, 0.1M potassium thiocyanate, 0.1M bis-Tris propane