6IRB

C-terminal coiled coil domain of Drosophila phospholipase C beta NORPA, selenomethionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	36.958, 37.591, 140.130
Unit cell angles	90.72, 89.86, 119.44

Refinement procedure

Resolution	14.904 - 2.661
R-factor	0.2276
Rwork	0.225
R-free	0.27980
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.091	0.065	0.827
Rmeas	0.108	0.077	0.984
Rpim	0.058	0.042	0.530
Total number of observations	59839
Number of reflections	17843	882	834
<I/σ(I)>	10.6
Completeness [%]	96.6	95.4	95.4
Redundancy	3.4	3.4	3.3
CC(1/2)		0.997	0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	7.2	289	64% 2-Methyl-2,4-pentanediol (MPD), 100mM HEPES, 8% Pentaerythritol ethoxylate (3/4 EO/OH)