6IR8
Rice WRKY/DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 48.225, 87.831, 99.691 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.916 - 2.300 |
| R-factor | 0.1955 |
| Rwork | 0.193 |
| R-free | 0.24440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aut |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.944 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.850 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.151 | 0.531 |
| Rpim | 0.024 | 0.105 |
| Number of reflections | 9748 | |
| <I/σ(I)> | 24.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.1 | |
| CC(1/2) | 0.997 | 0.972 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 278 | 0.27 M calcium acetate hydrate, 21% (w/v) PEG 3350 |
