6IQZ
High resolution structure of bilirubin oxidase from Myrothecium verrucaria - wild type
Replaces: 5ZD6Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-08 |
| Detector | RAYONIX MX225-HS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.342, 83.553, 52.690 |
| Unit cell angles | 90.00, 95.73, 90.00 |
Refinement procedure
| Resolution | 35.712 - 1.460 |
| R-factor | 0.1322 |
| Rwork | 0.131 |
| R-free | 0.16960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xll |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.127 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3235: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.490 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.088 | 0.691 |
| Number of reflections | 102516 | |
| <I/σ(I)> | 16.41 | 1.76 |
| Completeness [%] | 99.7 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1M SODIUM CITRATE PH 5.0, 20% (W/V) PEG 8000, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
