6IQH
X-ray crystal structure of covalent-bonded complex of Fc and peptide
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.082, 66.988, 78.336 |
Unit cell angles | 90.00, 106.41, 90.00 |
Refinement procedure
Resolution | 44.100 - 2.999 |
R-factor | 0.2269 |
Rwork | 0.223 |
R-free | 0.29270 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1dn2 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.633 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.100 | 3.110 |
High resolution limit [Å] | 2.999 | 3.000 |
Rmerge | 0.159 | 0.417 |
Number of reflections | 12253 | 1158 |
<I/σ(I)> | 8.4 | 1.6 |
Completeness [%] | 98.4 | 92.9 |
Redundancy | 3.1 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10 | 293 | 0.1 M CHES pH 10.0, 0.3M NaCl, 20% (w/v) PEG 8000 |