6IQ4

Nucleosome core particle cross-linked with a hetero-binuclear molecule possessing RAPTA and gold(I) 4-(diphenylphosphino)benzoic acid groups.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	98.15
Detector technology	PIXEL
Collection date	2017-09-18
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0398
Spacegroup name	P 21 21 21
Unit cell lengths	107.307, 109.741, 183.254
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.850 - 2.250
R-factor	0.23294
Rwork	0.232
R-free	0.25710
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.850	2.370
High resolution limit [Å]	2.250	2.250
Rmerge	0.051	2.340
Rpim	0.016	0.763
Number of reflections	102904
<I/σ(I)>	25.8
Completeness [%]	99.7	97.9
Redundancy	13	11
CC(1/2)	1.000	0.526

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291.15	manganese chloride, potassium chloride, potassium cacodylate