6IPC
Non-native human ferritin 8-mer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2018-10-01 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.304, 139.223, 139.114 |
| Unit cell angles | 90.10, 90.28, 90.18 |
Refinement procedure
| Resolution | 49.247 - 4.443 |
| R-factor | 0.2098 |
| Rwork | 0.207 |
| R-free | 0.27660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fha |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.789 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.247 | 4.602 |
| High resolution limit [Å] | 4.440 | 4.443 |
| Rmerge | 0.197 | |
| Number of reflections | 21483 | 495 |
| <I/σ(I)> | 2.3 | |
| Completeness [%] | 87.0 | 87 |
| Redundancy | 3.2 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293.15 | 0.1 M HEPES pH 7.5; 10% PEG 6000; 5% MPD |
