6IP3

Structure of human telomeric DNA at 1.4 Angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97949
Spacegroup name	P 6
Unit cell lengths	56.839, 56.839, 42.411
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.420 - 1.400
R-factor	0.1523
Rwork	0.151
R-free	0.17270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kf1
RMSD bond length	0.010
RMSD bond angle	0.990
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.100	0.880
Number of reflections	15493	777
<I/σ(I)>	16.4	3.5
Completeness [%]	100.0
Redundancy	16.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.05M sodium cacodylate (pH = 7.2), 0.4M ammonium sulfate, 0.05M KCl, 0.01M CaCl2, 15% PEG400