6IP0

Crystal structure of Arabidopsis thaliana JMJ13 catalytic domain in complex with AKG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.2824
Spacegroup name	P 62 2 2
Unit cell lengths	129.670, 129.670, 231.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.641 - 2.400
R-factor	0.1926
Rwork	0.192
R-free	0.21240
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.960
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.078	0.651
Rmeas		0.668
Rpim		0.151
Number of reflections	45790	4476
<I/σ(I)>	36.15	4
Completeness [%]	100.0	100
Redundancy	19.3	19.4
CC(1/2)		0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1M MES, pH 6.5, 8% dioxane, and 1.6M ammonium sulfate