6IP0
Crystal structure of Arabidopsis thaliana JMJ13 catalytic domain in complex with AKG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.2824 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 129.670, 129.670, 231.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.641 - 2.400 |
| R-factor | 0.1926 |
| Rwork | 0.192 |
| R-free | 0.21240 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.960 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.078 | 0.651 |
| Rmeas | 0.668 | |
| Rpim | 0.151 | |
| Number of reflections | 45790 | 4476 |
| <I/σ(I)> | 36.15 | 4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.3 | 19.4 |
| CC(1/2) | 0.945 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M MES, pH 6.5, 8% dioxane, and 1.6M ammonium sulfate |
