6IO9
The structure of apo-UdgX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-05 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.572, 50.958, 54.367 |
| Unit cell angles | 90.00, 104.56, 90.00 |
Refinement procedure
| Resolution | 36.606 - 1.900 |
| R-factor | 0.1917 |
| Rwork | 0.190 |
| R-free | 0.22520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ui0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.633 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.093 | 0.340 |
| Number of reflections | 15400 | 1486 |
| <I/σ(I)> | 22.6 | 4.7 |
| Completeness [%] | 97.2 | 97.1 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.980 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 298 | 0.05M MES 5.6, 2.45M (NH4)2SO4 and 0.1M MgCl2 |
