6INZ
Crystal structure of solute-binding protein complexed with unsaturated hyaluronan disaccharide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-07-28 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.740, 97.501, 132.443 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.410 - 2.289 |
R-factor | 0.1622 |
Rwork | 0.160 |
R-free | 0.21690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gub |
RMSD bond length | 0.022 |
RMSD bond angle | 1.645 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.289 | 2.289 |
Rmerge | 0.076 | 0.510 |
Number of reflections | 29129 | 1439 |
<I/σ(I)> | 23.6 | |
Completeness [%] | 99.9 | |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200mM litium sulfate, 100mM Tris-HCl (pH 8.5), 30% PEG 4000 |