6IN5
Crystal structure of H5N2 hemagglutinin G228S Q226L mutant with 3SLN from A/chicken/Taiwan/0502/2012
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 110.323, 110.323, 314.165 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.364 - 2.916 |
| R-factor | 0.2225 |
| Rwork | 0.219 |
| R-free | 0.29110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ykc |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.128 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.364 | 3.090 |
| High resolution limit [Å] | 2.916 | 2.910 |
| Rmerge | 0.084 | 0.718 |
| Rmeas | 0.094 | 0.804 |
| Rpim | 0.041 | 0.355 |
| Number of reflections | 16461 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 98.3 | 97.5 |
| Redundancy | 5.1 | 4.9 |
| CC(1/2) | 0.970 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1M Tris, 0.2M MgCl, 14%PEG8000 |
