6IN2
Crystal structure of BRD1 in complex with 18-Crown-6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 27.926, 59.641, 35.831 |
Unit cell angles | 90.00, 106.60, 90.00 |
Refinement procedure
Resolution | 29.758 - 1.750 |
R-factor | 0.2101 |
Rwork | 0.208 |
R-free | 0.24770 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.630 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1-2155_1069: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.800 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rpim | 0.041 | 0.343 |
Number of reflections | 11371 | |
<I/σ(I)> | 11.1 | |
Completeness [%] | 99.5 | |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 17% PEG4000, 0.1 M sodium acetate pH 4.4, 0.2 M ammonium acetate |